Dual-beam confocal light-sheet microscopy via flexible acousto-optic deflector
Confocal detection in digital scanned laser light-sheet fluorescence microscopy (DSLM) has been established as a gold standard method to improve image quality. The selective line detection of a complementary metal–oxide–semiconductor…
Heterodyne Fourier domain optical coherence tomography for full range probing with high axial resolution
One of the main drawbacks of Fourier domain optical coherence tomography (FDOCT) is the limited measurement depth range. Phase shifting techniques allow reconstructing the complex sample signal resulting in a…
Angular dispersion compensation for acoustooptic devices used for ultrashort-pulsed laser micromachining
Ultrashort pulsed laser material processing is a new micromachining method that is gaining interest. Its capability of submicrometer machining has been proved. To obtain high speed and highly flexible beam…
Acousto-optic lens with very fast focus scanning
We propose and experimentally demonstrate an acousto-optic cylindrical lens with a very fast (400-kHz) focal scanning. The lens is realized by use of two adjacent acousto-optic scanners with counterpropagating acoustic…
Multicolor Single-Molecule Spectroscopy with Alternating Laser Excitation for the Investigation of Interactions and Dynamics
We have developed confocal multicolor single-molecule spectroscopy with optimized detection sensitivity on three spectrally distinct channels for the study of biomolecular interactions and FRET between more than two molecules. Using…
Frequency-domain wide-field laser Doppler in vivo imaging
We present a new instrument, based on a low-frame-rate 8Hz CCD camera used in a heterodyne opticalmixing configuration, that can create wide-field laser Doppler maps. As an illustration, we show…
Q-Switches (french article)
Les lasers dits Q-switchés occupent une large place dans la famille des lasers impulsionnels. Cette technique de génération d’impulsions permet en effet d’obtenir de fortes puissances crêtes, indispensables dans de…
Basic building units and properties of a fluorescence single plane illumination microscope
The critical issue of all fluorescence microscopes is the efficient use of the fluorophores, i.e., to detect as many photons from the excited fluorophores as possible, as well as to…
Development of an atom gravimeter and status of the 10-meter atom interferometer for precision gravity measurement
Experimental realizations of cold 85Rb atom interferometers in Wuhan are reviewed in this paper. The application of atom interferometers in local gravity measurement are reported. The resolutions of gravity measurement…
Fast two-photon in vivo imaging with three-dimensional random-access scanning in large tissue volumes
the understanding of brain computations requires methods that read out neural activity on different spatial and temporal scales. Following signal propagation and integration across a neuron and recording the concerted…
Interference and crosstalk in double optical tweezers using a single laser source
Experimental studies of single molecule mechanics require high force sensitivity and low drift, which can be achieved with optical tweezers. We built an optical tweezer setup for force measurements in…
Ultrafast random-access scanning in two-photon microscopy using acousto-optic deflectors
Two-photon scanning microscopy (TPSM) is a powerful tool for imaging deep inside living tissues with sub-cellular resolution. The temporal resolution of TPSM is however strongly limited by the galvanometric mirrors…
Frequency-domain wide-field laser Doppler in vivo imaging
We present a new instrument, based on a low-frame-rate 8Hz CCD camera used in a heterodyne opticalmixing configuration, that can create wide-field laser Doppler maps. As an illustration, we show…
Quantum Dot Photon Statistics
We present an instrument for performing correlation spectroscopy on single fluorescent particles while tracking their Brownian motion in three dimensions using real-time feedback. By tracking CdSe/ZnS quantum dots in water…
High-speed in vivo calcium imaging reveals neuronal network activity with near-millisecond precision
two-photon calcium imaging of neuronal populations enables optical recording of spiking activity in living animals, but standard laser scanners are too slow to accurately determine spike times. here we report…
Millijoule pulse energy Q-switched short-length fiber laser
We report on a Q-switched short-length fiber laser producing 100 W of average output power at 100 kHz repetition rate and pulse durations as short as 17 ns.Upto2mJof energy and…
Double-pass acousto-optic modulator system
A practical problem that arises when using acousto-optic modulators sAOMsd to scan the laser frequency is the dependence of the beam diffraction angle on the modulation frequency. Alignment problems with…
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